Assay for testing effect of compound on Ser910/ Ser 935 phosphorylation of LRRK2, and/or binding of 14-3-3 polypeptide

摘要

A method for assessing the effect of a test compound on LRRK2 in a cell-based system, the method comprising the steps of a) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the phosphorylation state of Ser910 and/or Ser935 of the LRRK2; and/or c) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the binding of the LRRK2 to a 14-3-3 polypeptide. The method is considered to be useful in assessing the effect of putative LRRK2 inhibitors in cell based systems, including in vivo systems. Kits comprising antibodies specific for the phophorylated and/ or dephosphorylated Ser 910 and/ or Ser935, and antibodies to 14-3-3 are also provided.