FLUORESCENCE DETECTING DEVICE AND FLUORESCENCE DETECTING METHOD

摘要

A fluorescence detecting device receives fluorescence emitted by n kinds of measurement objects within wavelength bands FL k (k is an integer of 1 to n) set so that the fluorescence intensity of fluorescence emitted by a measurement obj ect k becomes higher than that of fluorescence emitted by the other one or more measurement objects, and acquires fluorescent signals corresponding to the wavelength bands FL k (k is an integer of 1 to n). Each of the fluorescent signals is subjected to frequency-down conversion by mixing it with a modulation signal for modulating the intensity of a laser beam L k (k = 1) corresponding to at least one of the wavelength bands FL k to produce fluorescence data including the phase delay and intensity amplitude of the fluorescent signal. The fluorescence data is corrected to calculate the phase delay and a fluorescence relaxation time is calculated using the phase delay.