METHOD FOR PRODUCING TOTAL WATER-SOLUBLE PROTEOLYTIC ENZYMES

摘要

FIELD: medicine. ^ SUBSTANCE: method for producing total water-soluble proteolytic enzymes from dried milky sap of green papaya fruits provides water extraction of a raw material at room temperature and with mixing, separation of solid and liquid phases, and recovery of a purified product. An aqueous extract produced after separation of the solid and liquid phases is post-filtered through a filter of pore size (0.45-2.0) mcm. The product is dried by a spraying technique to prepare a purified product in the form of a dry extract of proteolytic activity not less than 3.5 PU/mg of the preparation and the protein content 0.43-0.50 mg/mg of the preparation. The prepared dry extract is water-dissolved that is followed by sterilisation filtration and lyophilisation of the solution. ^ EFFECT: method allows preparing a product of high proteolytic activity and protein content. ^ 2 cl, 1 ex