发明名称 |
A COMPLEX AND METHOD FOR ENHANCING NUCLEAR DELIVERY |
摘要 |
The use of at least one nucleic acid based nuclear localization signal including a natural or synthetic m3G-CAP is shown to increase transmembrane transport of a molecular cargo, in particular large molecules, into the nucleus. The use of natural and synthetic m3G-CAP is disclosed and the effect shown with a natural RNA 5′ end nuclear localization signal composed of a 2,2,7-trimethylguanosine CAP (m3G-CAP) coupled to fluorescent Streptavidin in one example, and an antisense oligonucleotide in another. A methylenephosphonate modified m3G-CAP is shown to have improved stability in human serum and in cytosolic extract. |
申请公布号 |
EP2376518(A1) |
申请公布日期 |
2011.10.19 |
申请号 |
EP20090833750 |
申请日期 |
2009.12.17 |
申请人 |
AVARIS AB |
发明人 |
SMITH, EDVARD;MORENO, PEDRO;STROEMBERG, ROGER;WENSKA, MALGORZATA |
分类号 |
C07H19/167;A61K31/7115;A61K31/712;C07H19/20;C07H19/207;C07H21/02 |
主分类号 |
C07H19/167 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|