发明名称 |
METHOD FOR EVALUATING SPECIFIC INCORPORATION OF D-GLUCOSE INTO CELLS |
摘要 |
<p>An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.</p> |
申请公布号 |
EP2325327(A4) |
申请公布日期 |
2011.10.19 |
申请号 |
EP20090805069 |
申请日期 |
2009.08.07 |
申请人 |
HIROSAKI UNIVERSITY;NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY |
发明人 |
YAMADA KATSUYA;MATSUOKA HIDEAKI;TESHIMA TADASHI;YAMAMOTO TOSHIHIRO |
分类号 |
C12Q1/54;C07H1/00;C07H5/06;C07H13/00;C07H17/00;C12Q1/02;C12Q1/04;G01N33/50 |
主分类号 |
C12Q1/54 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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