METHOD AND KIT FOR SEPARATING VIRAL AND PROKARYOTIC NUCLEIC ACIDS FROM EUKARYOTIC NUCLEIC ACIDS

摘要

The invention relates to a method for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample, in particular a eukaryotic cell suspension, comprising the following steps in the following order: a) resuspending the cells in the presence of a chelate binder, in particular EDTA or EDTA in combination with a saccharide, b) lysis of the cells by chemical lysis, such as alkaline lysis, enzymatic lysis and/or boiling lysis and/or mechanical lysis, c) neutralizing the cell lysate and d) separating the precipitated eukaryotic nucleic acid and obtaining the viral and/or prokaryotic nucleic acids. The invention further relates to the use of a kit comprising a resuspension buffer having a chelate binder and optionally a saccharide and RNAse, lysis buffer comprising at least one base and a detergent and neutralization buffer to at least partially separate viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample, in particular a eukaryotic cell suspension.