METHOD FOR SELECTIVE ISOLATION AND PURIFICATION OF NUCLEIC ACIDS

摘要

The present invention relates to a gentle method for isolating and purifying nucleic acids from a biological cell comprising sample comprising at least DNA, RNA, and proteins, comprising at least the steps of 1. mixing the sample with a lysis buffer, 2. incubating the sample at a temperature within a range between 45°C and 59°C to obtain DNA as well as RNA or within a range between 60°C and 70°C, to obtain DNA essentially free of RNA and 3. separating the nucleic acids from any contaminants.