发明名称 |
PCR-BASED METHOD OF SYNTHESIZING A NUCLEIC ACID MOLECULE |
摘要 |
<p>There is provided a method of synthesizing a nucleic acid molecule and in one aspect, the method comprises assembling a full length template nucleic acid molecule by PCR in a PCR reaction mixture comprising a set of assembly oligonucleotides having a first average melting temperature and a set of outer amplification primers having a second average melting temperature that is lower than the first average melting temperature, wherein said assembling comprises subjecting the PCR reaction mixture to a first annealing temperature that is higher than the second average melting temperature and; amplifying the full length template nucleic acid molecule by PCR in the PCR reaction mixture wherein said amplifying comprises subjecting the PCR reaction mixture to a second annealing temperature that permits annealing of the outer amplification primers to the full length template nucleic acid molecule.</p> |
申请公布号 |
EP2373807(A1) |
申请公布日期 |
2011.10.12 |
申请号 |
EP20080879001 |
申请日期 |
2008.12.19 |
申请人 |
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH |
发明人 |
LI, MO-HUANG;YING, JACKIE Y.;YE, HONGYE |
分类号 |
C12Q1/68;C07H21/00;C12N15/10;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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