| 摘要 |
The present invention provides methods of multiplying plant embryogenic tissue in a bioreactor. The method of multiplying plant embryogenic tissue in a bioreactor specifically comprisies the steps of: (a) inoculating plant embryogenic tissue and multiplication media into a bioreactor; (b) measuring a parameter that is a direct measure of, or correlated to, a concentration of the plant embryogenic tissue in the bioreactor; (c) using the measurement obtained in step (b) to calculate a flow rate of additional multiplication media; (d) delivering additional multiplication media to the bioreactor based on the flow rate calculated in step (c); and (e) periodically repeating steps (b) to (d). |
