| 摘要 |
PROBLEM TO BE SOLVED: To manufacture a hyaluronic acid with high purity and pharmaceutical grade in which an exothermic substance, a protein, a microorganism and fine particles are removed and/or its salt (hereinafter referred to as HA) at a high yield and in an industrial scale.SOLUTION: The purification method for the hyaluronic acid and/or its salt includes: a step A for bringing a liquid containing HA into contact with an inorganic adsorbent or an inorganic adsorbent and an activated carbon, a step B for performing dialysis treatment by an ultrafiltration membrane after the liquid containing HA is adjusted to a pH range at an acidic side, a step C for bringing the liquid containing HA into contact with a ceramic membrane, step D for bringing the liquid containing HA into contact with a filler for chromatography making a natural substance as a base material or a filler for reverse phase chromatography in which a substituted or unsubstituted hydrocarbon group having chain length of 8 or more of C is bonded to a carrier, and step E for bringing the liquid containing HA into contact with a microfiltration membrane. |
