摘要 |
Disclosed is an oligonucleotide analogue comprising a consecutive sequence of n nucleotides and/or nucleotide analogues and at least two hydrophobic nucleotides, wherein said oligonucleotide analogue is covalently linked to a signalling pair, wherein the hydrophobic nucleotide has the general structure: X-Y-Q wherein: X is a nucleotide or nucleotide analogue or a backbone monomer unit capable of being incorporated into the backbone of a nucleic acid or nucleic acid analogue, Q is a hydrophobic molecule which is not taking part in Watson-Crick hydrogen bonding; and Y is a linker moiety linking said nucleotide or nucleotide analogue or backbone monomer unit and said hydrophobic molecule; and wherein n is an integer of at least 4; and wherein the at least two hydrophobic nucleotides are positioned at the most 9 nucleotides and/or nucleotide analogues from one end; and wherein the at least two hydrophobic nucleotides are positioned in approximately the same distance from the respective parts of the signalling pair, and wherein the signalling pair consists of a two part system, wherein one part is positioned at the most 6 nucleotides and/or nucleotide analogues from the 5' end and the other part is positioned at the most 6 nucleotides or nucleotide analogues from the 3' end; wherein the parts of the signalling pair are not identical to the hydrophobic nucleotide, with the proviso that when one part of the signalling pair is positioned as a dangling end at the 5' end, then the first nucleotide or nucleotide analogue at the 5' end is not a hydrophobic nucleotide and when one part of the signalling pair is positioned as a dangling end at the 3' end, then the first nucleotide or nucleotide analogue at the 3' end is not a hydrophobic nucleotide.
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