Cyclic protein free from cysteines

摘要

Disclosed is a protein selected from the amino acid sequence of the region valine Val(91) to glycine Gly(121) of the mature human tumour necrosis factor, or a portion thereof, with the proviso that the protein comprises at least the amino acid sequence of the region lysine Lys(98) to glutamic acid Glu(116), with the cysteine Cys(101) being replaced by a glycine and an amide bond being formed between the amino group of the side chain of the lysine Lys(98) and the carboxyl group of the side chain of the glutamic acid Glu(116), wherein the mature human tumour necrosis factor is of the following amino acid sequence SEQ ID NO:3: (NH2)Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu (COOH). Further disclosed is the use of said proteins in the preparation of a medicament for the treatment of diseases associated with the lung function, pulmonary oedemas and for the prevention of inflammation. Particular disclosed proteins as disclosed comprise the amino acid sequence VNLLSAIKSP CQRETPEGAEAKPWYEPIYLG wherein an amide bond is formed between the amino group of the side chain of Lysine(6) and the carboxyl group of the side chain of glutamic acid(26) or KSPCQRETPEGAEAKPWYE wherein an amide bond is formed between the amino group of the side chain of Lysine(1) and the carboxyl group of the side chain of glutamic acid(19).