| 摘要 |
Disclosed is a liquid test strip comprising a substrate having at least one channel thereon, the channel having a first region, a second region and a third region that are arranged successively, the first region being used for a fluid sample to be introduced there into. The liquid test strip is characterized by: a first antibody localized in the first region for recognizing an analyte in the fluid sample; a saccharide localized in the first or the second region; a peroxidase localized in the first or the second region; a second antibody immobilized in the second region for recognizing the analyte in the fluid sample, and the second antibody and the first antibody being configured to recognize different epitopes of the analyte in the fluid sample; and an optical substrate and a substrate reagent, both localized in the third region, wherein the substrate reagent comprises a saccharide oxidase. Upon introduction of the fluid sample containing the analyte into the channel, the first antibody, the saccharide, and the peroxidase flow along with the fluid sample, a part of the peroxidase binds with the first antibody, the analyte and the second antibody so as to be retained in the second region allowing the other part of the peroxidase to flow along with the fluid sample to the third region. The saccharide arrives at the third region and is catalysed by the saccharide oxidase so as to produce hydrogen peroxide. The optical substrate is catalysed by the peroxidase arriving at the third region so as to react with the hydrogen peroxide to generate an optical signal. Also disclosed is a method of detecting the analyte using the liquid test strip.
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