发明名称 |
METHOD OF PRODUCING RECOMBINANT PROTEINS WITH MANNOSE- TERMINATED N-GLYCANS |
摘要 |
We describe a method of expressing a recombinant protein comprising mannose- terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-1 into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose- terminated glycan structure, suitable for treatment or prevention of Gaucher' s Disease. |
申请公布号 |
WO2011119115(A1) |
申请公布日期 |
2011.09.29 |
申请号 |
WO2011SG00118 |
申请日期 |
2011.03.24 |
申请人 |
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH;SONG, ZHIWEI |
发明人 |
SONG, ZHIWEI |
分类号 |
C12N5/10;C07K14/005;C07K14/47;C12N5/071;C12N15/12;C12N15/33;C12N15/56;C12N15/85 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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