CULTURE METHOD FOR CAUSING DIFFERENTIATION OF PLURIPOTENT MAMMALIAN CELLS

摘要

Disclosed is a method that, using a cell culture vessel wherein a plurality of microvessels have been formed, achieves control over embryoid body size and can induce differentiation in the state wherein embryoid body size is controlled. The culture method that causes the differentiation of pluripotent mammalian cells uses a cell culture vessel (10) having a plurality of microvessels (11) on the culture surface. The cell culture vessel (10) has a culture surface configured from spaces of which the height of the spatial structure of the microvessels (11) is 10-500 µm and the floor area is 100 µm2-1 mm2. Using the cell culture vessel (10), the culture method that causes the differentiation of pluripotent mammalian cells cultures pluripotent mammalian cells and obtains cell aggregates that are at least partially differentiated into endoderm lineage cells.