DEVICES, SYSTEMS, AND METHODS FOR AMPLIFYING NUCLEIC ACIDS

摘要

The present invention generally relates to a devices, systems, and methods for amplifying nucleic acids in flowing droplets. In certain embodiments, the invention provides a device for amplifying nucleic acids including at least one channel through which sample droplets including nucleic acids flow, in which the nucleic acids in the droplets are optically detectable while the droplets are flowing through the channel, and a plurality of temperature zones in thermal contact with the channel, in which the zones are located at different locations along the channel and the zones are separated from each other.