| 摘要 |
<p>The invention relates to the production of recombinant peptides and proteins in eukaryotic cells and organisms as host systems after transformation of the host with an appropriate vector. A target polypeptide is fused to a synthetic mitochondrial localising sequence and expressed as a fusion product, said fusion product being driven to the mitochondria mediated by the mitochondrial localisation sequence. There the fusion polypeptide is broken to release the product of interest and the mitochondrial localising sequence; the mitochondrial localisation sequence is specifically removed enzymatically by mitochondrial processing enzymes. The target polypeptide is then stored inside the mitochondria and accumulates in the host cell or organism. The isolation and accumulation within the mitochondria allows for protection for the product of interest from the cellular environment, and also for protection of the cell from the product of interest.</p> |
