| 摘要 |
The present invention relates to the use of a signal peptide for producing a recombinant polypeptide of interest in an expression system, wherein said signal peptide comprises at least 12 amino acids of formula (I): (X1)iX2X3X4SX5X6X7, in which: -X1 is a peptide containing from 3 to 6 amino acids, i is equal to 0 or 1, -X2 is a peptide containing from 3 to 9 hydrophobic amino acids, -X3 is a peptide containing from 3 to 5 amino acids, said peptide comprising at least 3 contiguous or non-contiguous leucines, -X4 is a peptide containing from 2 to 5 amino acids selected from Ala, Thr, Ser, Gln, Ile and Met, -X5 is Ala or Val, -X6 is Gln, Asn or His, and -X7 is Ala or Cys, with the proviso that, when said signal peptide results from a natural precursor of a predetermined protein, said polypeptide of interest is different from said protein. |
