| 摘要 |
Primer sets for amplifying target regions containing sites to be detected in the obesity gene (the &bgr;2AR gene, the &bgr;3AR gene, and the UCP1 gene) by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers composed of the base sequences of SEQ ID NO: 9 or SEQ ID NO: 109, SEQ ID NO: 25, and SEQ ID NO:43 as well as reverse primers composed of the base sequences of SEQ ID NO: 18, SEQ ID NO: 30, and SEQ ID NO: 63, respectively. The use of these primer sets makes it possible to specifically amplify a target region including a site where a polymorphism to be detected is generated in the &bgr;2AR gene, the &bgr;3AR gene, and the UCP1 gene, in the same reaction solution at the same time.
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