发明名称 |
Method for preparing oligonucleotides |
摘要 |
A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large- scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3′-protected compound of formula: with a nucleotide derivative having a 5′-protection group, or reacting a 5′-protected compound of formula with a nucleotide derivative having a 3′-protection group.
|
申请公布号 |
US2011224424(A1) |
申请公布日期 |
2011.09.15 |
申请号 |
US201113115845 |
申请日期 |
2011.05.25 |
申请人 |
GIRINDUS AG;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;UNIVERSITY OF MONTPELLIER II |
发明人 |
ADAMO ILARIA;DUEYMES CECILE;SCHOENBERGER ANDREAS;IMBACH JEAN-LOUIS;MEYER ALBERT;MORVAN FRANCOIS;DEBART FRANCOISE;VASSEUR JEAN-JACQUES;LANGE MEINOLF;LINK FRITZ |
分类号 |
C07H1/00;C07H11/04;C07H21/00 |
主分类号 |
C07H1/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|