SPECIFIC DETECTION OF HUMAN CHORIONIC GONADOTROPIN BETA SUBUNIT TYPE II PRODUCED BY TROPHOBLASTIC AND NEOPLASTIC CELLS

摘要

The present invention provides a novel method to distinguish between HCG ß type I and type II gene expression using specific antibody. The specific recognition of HCGß encoded by type II genes and expressed by trophoblastic and neoplastic cells might improve the clinical usefulness of assays aimed at either diagnosing tumors or screening Down's syndrome. The present invention also provides a diagnostic kit for determining the amount of HCGß type II in a biological sample. The present invention additionally provides process of preparation and screening hybridoma capable of specifically recognizing HCGß type II and recombinant antibody thereof. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.