VERFAHREN ZUM WACHSTUM VON MENSCHLICHEN BINDEHAUTGEWEBEÄQUIVALENTEN FÜR DIE FORSCHUNG, DIE KLINISCHE AUGENOBERFLÄCHENTRANSPLANTATION UND DIE GEWEBEREKONSTRUKTION

摘要

The invention relates to materials and methods for producing equivalents of tissues of the ocular surface, especially conjunctival tissue equivalents. The method of the invention involves biopsy of an appropriate tissue, primary culture in a certain medium, proliferative culture in a second medium, and differentiative culture in a third medium. The tissue equivalents are typically grown upon a substrate, typically amniotic membrane, which provides ease of handling as one advantage.