METHODS AND REAGENTS FOR ENRICHMENT AND CHARACTERIZATION OF PHOSPHORYLATED BIOMOLECULES

摘要

An affinity matrix comprising a metal ion covalently attached thereto and methods for making and using the same are described. The matrix has affinity for various phosphorylated biomolecules, such as phosphoproteins/phosphopeptides. The matrix may be used in a variety of different applications, including phospho-biomolecule (e.g., phosphoprotein and phosphopeptides) enrichment/purification and characterization applications. Also provided are kits and systems that include the matrix.