LATERAL FLOW DEVICE AND METHOD OF DETECTION OF NUCLEIC ACID SEQUENCE

摘要

The present invention relates to a lateral flow device (1) for detecting the presence or absence of at least one target nucleic acid sequence (13) bearing a first hapten label (14) and a second hapten label (15), comprising a) a conjugates region (3) containing a detector conjugate (9) which binds to the second hapten label (15) of the target nucleic acid sequence (13) and is provided with a signalling label; b) an application region (2) adjacent to said conjugates region (3); c) a detection region (4) adjacent to said application region (2), said detection region (4) containing at least one capture sub-region (6) containing a capture reagent (10) immobilized in the capture sub-region (6), which binds to the first hapten label (14) of the target nucleic acid sequence (13); d) an adsorbent region (5) adjacent to said detection region. Further, it provides a chromatographic control of the workability of the device and for an internal amplification control that verifies a nucleic acid amplification assay. In addition to that, the present invention relates to a method using said device.