发明名称 |
NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFORE |
摘要 |
[Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.
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申请公布号 |
US2011224417(A1) |
申请公布日期 |
2011.09.15 |
申请号 |
US201113089420 |
申请日期 |
2011.04.19 |
申请人 |
KANKYO ENGINEERING CO., LTD.;NATIONAL INSTITUTE OF ADV. INDUSTRIAL SCI. & TECH. |
发明人 |
NAKAMURA KAZUNORI;KANAGAWA TAKAHIRO;NODA NAOHIRO;TSUNEDA SATOSHI;TANI HIDENORI;KURATA SHINYA |
分类号 |
C07H21/04;C12Q1/68;G01N21/64;G01N33/53;G01N33/58 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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