发明名称 |
Method for chondrocyte expansion with phenotype retention |
摘要 |
The present invention provides a method for expanding a population of chondrocytes that maintains chondrocyte phenotype during the expansion by culturing the population in a defined serum-free expansion medium containing one or more cytokines and under low attachment conditions. The method further solves diffusion problems during the subsequent stage of extracellular matrix production by use of a perforated polycarbonate substrate that results in a randomly organized cultured neocartilage tissue. Chondrocytes expanded and cultured in this manner can be used in various medical applications to repair cartilaginous tissues that have been injured by trauma or disease.
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申请公布号 |
US8017394(B2) |
申请公布日期 |
2011.09.13 |
申请号 |
US20070859524 |
申请日期 |
2007.09.21 |
申请人 |
ISTO TECHNOLOGIES, INC. |
发明人 |
ADKISSON, IV HUSTON DAVIS;MILLIMAN CURT L;KIZER NEIL |
分类号 |
C12N5/00;C12N5/077 |
主分类号 |
C12N5/00 |
代理机构 |
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地址 |
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