| 摘要 |
FIELD: medicine. ^ SUBSTANCE: what is described is a diagnostic technique for deletions and duplications of PARK2 gene exons involving simultaneous amplification of one of park gene exons and a tracking gene in test samples of human genome DNA by real-time polymerase chain reaction with using exon-flanking outer primers and located between primers of fluorescent-marked hybridisation probes where polymerase chain reaction is four-staged in the following conditions: a reaction mixture is heated at temperature 50C for 2 minutes, denatured at 95C for 15 minutes, denatured at 95C for 15 seconds, incubated at 61C for 45 seconds; 35 cycles are repeated consistently with the two last stages; the absence of deletions, the presence of heterozygotic or homozygous deletions or the presence of duplications of exon is stated by the coefficient R which is calculated by formula R=2-(öCt), where öCt = [Ct tracking gene (reference DNA) - Ct park (reference DNA)] - [Ct tracking gene (patient's DNA sample)-Ct park PARK2 (patient's DNA sample)]. ^ EFFECT: invention allows detecting mutations in PARK2 gene. ^ 14 cl, 5 tbl, 2 ex |
