METHODS OF MEASURING PROTEIN STABILITY

摘要

A method of determining a degradation rate of a fluorescently labeled polypeptide of interest in a population of cells is provided. The method comprises: (a) obtaining two identical populations of cells; (b) determining fluorescence levels of the fluorescently labeled polypeptide of interest in a first of the at least two populations of cells at at least two different points in time; (c) at least partially reducing a fluorescence of the fluorescently labeled polypeptide of interest in a second of the at least two populations of cells; (d) determining fluorescence levels of the reduced fluorescently labeled polypeptide of interest in the second of said at least two populations of cells at said at least two different points in time; (e) comparing fluorescence levels in the first and the second populations; and (f) based on thee comparing, determining a degradation rate of the fluorescently labeled polypeptide of interest.