IMPROVED BIOREACTOR PERFORMANCE IN THE PRODUCTION OF MONATIN
摘要
Methods and systems for increasing the production of monatin in a multi-step equilibrium pathway are described. Tryptophan and pyruvate are added to a bioreactor to form a mixture comprising monatin and a plurality of intermediates via a multi-step equilibrium pathway. The methods and systems include operating the bioreactor such that a temperature of the mixture in the bioreactor is less than 25 degrees Celsius, resulting in an increased production of monatin. In some embodiments, the temperature of the mixture in the bioreactor is between about 5 degrees Celsius and about 23 degrees Celsius; in other embodiments, the temperature is between about 10 degrees Celsius and about 18 degrees Celsius.
申请公布号
WO2011082365(A3)
申请公布日期
2011.09.09
申请号
WO2010US62603
申请日期
2010.12.30
申请人
CARGILL, INCORPORATED;MARASCO, ERIN;PEMBLE, TRENT;PORTER, MICHAEL A.;RASMUSSEN, MARIBETH;SOLHEID, CHRISTOPHER
发明人
MARASCO, ERIN;PEMBLE, TRENT;PORTER, MICHAEL A.;RASMUSSEN, MARIBETH;SOLHEID, CHRISTOPHER