STABLE INTEGRATION OF A XENOBIOTIC RECEPTOR PROMOTER-REPORTER DNA CONSTRUCT INTO A HUMAN LIVER CELL LINE FOR EVALUATING THE DRUGS BY HIGH THROUGHPUT SCREENING

摘要

The present invention relates to a stably integrated pregnane and xenobiotic receptor (PXR) promoter-reporter construct-cell line comprising PXR promoter of -1096 to +43 region having sequence listing as illustrated in Figure 1, which is capable of assessing drug's capability to bind and induce PXR-promoter on its administration, and hence, is capable of indirectly influencing the protein level of the PXR-promoter on its administration so as to avoid failure of combination therapy. In one embodiment, it relates to a method of preparation of stably integrated pregnane and xenobiotic receptor (PXR) promoter-reporter construct-cell line. In another embodiments, it relates to a pregnane and xenobiotic receptor (PXR) promoter and to a pregnane and xenobiotic receptor (PXR)-promoter-reporter construct.