METHOD FOR PRODUCING RECOMBINANT HUMAN FSH

摘要

PROBLEM TO BE SOLVED: To provide a method for refining a recombinant human FSH (Follicle-Stimulating Hormone) in high yield and high purity from a culture fluid of recombinant human FSH-producing cells using only steps without use of any organic solvents. SOLUTION: The method for producing the recombinant human FSH comprises: the step of (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium and secreting a recombinant human FSH in the culture fluid; the step of (b) preparing a culture supernatant; the step of (c) subjecting the culture supernatant to cation-exchange column chromatography to retrieve a human FSH-active fraction; the step of (d) subjecting the fraction to dye affinity column chromatography to retrieve a human FSH-active fraction; the step of (e) subjecting the fraction to hydrophobic column chromatography to retrieve a human FSH-active fraction; and then the step of (f) subjecting the fraction to gel filtration column chromatography to retrieve the final recombinant human FSH-active fraction. COPYRIGHT: (C)2011,JPO&INPIT