| 摘要 |
An apparatus and a method of locating a specimen with a microscope, the microscope including two point light sources, a transparent specimen holder, a digital imaging device, a processor and a display. The method includes sequentially illuminating the two point light sources through the transparent specimen holder and across the specimen and onto the digital imaging device to create shadows associated with each of the two point light sources. Optical images of the specimen are received on the digital imaging device associated with each shadow from the two point light sources, and a disparity signal is output containing a horizontal position, a vertical position and a depth position of the specimen in the transparent specimen holder relative to the digital imaging device. A location of the specimen is determined relative to the microscope based on the disparity signal, where the transparent specimen holder intersects a unidirectional path of light from each point light source of the two point light sources to the digital imaging device and allows the specimen to move freely throughout the transparent specimen holder.
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