发明名称 |
METHOD FOR SCREENING NUCLEIC ACID LIGAND |
摘要 |
<p>Provided is a method for screening a library of candidate for a nucleic acid ligand. The method includes the steps of: (a) preparing a library of candidate of nucleic acid ligands; (b) contacting, under the absence of a target substance, the library with a supporting member binding to at least one of conserved sequence domains included in the ligand, and then separating and removing a ligand which does not form an intermolecular duplex; and (c) dissociating the intermolecular duplex by contacting, under the presence of the target substance, the target substance with the remaining ligand forming the intermolecular duplex obtained in step (b), and then separating and collecting a ligand having a specific secondary structure formed by the binding to the target substance, wherein the method includes at least one time of step (b) and at least one time of step (c).</p> |
申请公布号 |
WO2011105071(A1) |
申请公布日期 |
2011.09.01 |
申请号 |
WO2011JP01024 |
申请日期 |
2011.02.23 |
申请人 |
CANON KABUSHIKI KAISHA;HATAKEYAMA, SATORU;IBII, TAKAHISA |
发明人 |
HATAKEYAMA, SATORU;IBII, TAKAHISA |
分类号 |
C12Q1/68;C12N15/09;G01N33/53 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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