USING PHYLOGENETIC PROBES FOR QUANTIFICATION OF STABLE ISOTOPE LABELING AND MICROBIAL COMMUNITY ANALYSIS

摘要

Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).