发明名称 Polyclonal-monoclonal ELISA assay for detecting N-terminus proBNP
摘要 <p>A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides; Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.</p>
申请公布号 EP2251356(A3) 申请公布日期 2011.08.31
申请号 EP20100169301 申请日期 2003.11.17
申请人 NEXUS DX, INC. 发明人 DAVEY, MICHELLE;JACKOWSKI, GEORGE;KUPCHAK, PETER;STANTON, ERIC
分类号 C07K16/26;G01N33/68;G01N33/74 主分类号 C07K16/26
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