| 摘要 |
Disclosed is a method for detecting a bacterium that produces a cell-wall-synthesizing enzyme, PBP2'. The method comprises detection of a cell-wall-synthesizing enzyme PBP2' via immunochromatography detection based on an antigen-antibody reaction using an antibody that specifically detects PBP2'. The method involves the use of an immunochromatography detection device comprising on a sheet-like solid-phase support; a sample supply site to which a sample solution deduced to contain a bacterium producing a cell-wall-synthesizing enzyme, PBP2' or a solution deduced to contain PBP2' released from the cell wall via sample pre-treatment is supplied; a labelled reagent site that holds a labelled reagent which comprises a labelled antibody binding specifically to PBP2', such that the reagent is able to spread across the solid-phase support; and a capture reagent site on which a capture reagent capable of specifically binding to and capturing a complex of PBP2' by capturing the antibody and the labelled reagent that comprises a labelled antibody has been immobilized, wherein the capture reagent site comprises a sulfobetaine-type surfactant.
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