EXPRESSION OF IMMUNOGLOBULIN-CYTOKINE FUSION PROTEINS IN MALIGNANT B CELLS

摘要

According to the invention, there is provided a vector for the expression of immunoglobulin-cytokin fusion proteins in malignant B cells at least containing operably linked to each other (a) a region of at least 1.5 kb which is homologous to a region of the μ intron or the &kgr; intron and which lacks a functional Cμ or C&kgr; enhancer or contains a non-functional Cμ or C&kgr; enhancer; (b) at least one DNA sequence encoding a domain of an immunoglobulin or a part thereof; (c) a DNA sequence encoding a cytokin; and (d) a marker gene selectable in eukaryotic B cells and lacking a functional enhancer region wherein the expression of said marker following integration is controlled by the cellular Cμ or C&kgr; enhancer.