| 摘要 |
<p>Disclosed is a reporter gene which, in an analysis method using a reporter gene such as the reporter gene assay method or the two-hybrid assay method, makes it possible to shorten the external stimulus time to the cell, and can make it possible for the intensity of luminescence caused by the reporter gene expression in response to the external stimulus to quantitatively and accurately reflect said degree of external stimulus. In the analysis method for analyzing the amount or timing of target gene expression in a cell in response to an external stimulus, at least one of the amino acid residues at positions corresponding to positions 89 to 118 in the amino acid sequence of Gaussia Luciferase (GLuc) is replaced in the amino acid sequence of a luminescent enzyme of a marine animal as a reporter gene, said replacement being a conservative amino acid replacement of the amino acid residue(s) in at least one position chosen from the positions corresponding to positions 89, 90, 95, 97, 100, 108, 112, 115 and 118 in said amino acid sequence, and a gene is used encoding the altered luminescent enzyme with thusly improved luminescence.</p> |
