METHOD FOR CONTROLLING pH, OSMOLALITY AND DISSOLVED CARBON DIOXIDE LEVEL IN MAMMALIAN CELL CULTURE PROCESS TO ENHANCE CELL VIABILITY AND BIOLOGIC PRODUCT YIELD.

摘要

PROBLEM TO BE SOLVED: To provide methods for controlling the level of dissolved carbon dioxide and limiting the osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield.

SOLUTION: The control of the dissolved carbon dioxide level and pH, as well as the resulting ability to limit osmolality in a mammalian cell culture process are achieved by adopting alternative pH control strategies and CO2stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping are carried out without generating foam and with little or no damage to the mammalian cells.

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