发明名称 |
PARALLEL NUCLEIC ACID SEQUENCING METHODS |
摘要 |
The present invention relates to a method of sequencing a target polynucleotide by enzymatic and/or chemical means. The sequencing method includes a method for characterizing multiple alleles in a sample, a method for comparing polynucleotide sequences. The method involves analysing molecules as members of an array. Many target polynucleotides or many segments of a single target polynucleotide can be sequenced simultaneously. In a preferred embodiment the method involves analysing individual molecules within an array and base calls are based on the signals from two or more molecules. A method to prevent non-specific signal in sequencing is also provided. The invention is readily automated, both for small-scale and large-scale operation and relevant algorithms and the composition of kits and systems are provided. |
申请公布号 |
EP1689881(B1) |
申请公布日期 |
2011.08.24 |
申请号 |
EP20040768958 |
申请日期 |
2004.10.20 |
申请人 |
ISIS INNOVATION LIMITED |
发明人 |
SHCHEPINOV, MIKHAIL, S.;MIR, KALIM |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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