RECOMBINANT MULTIPLEX POLYMERASE CHAIN REACTION PRIMER DETECTING DIARRHOEAL TYPED TOXIN AND EMETIC TYPED TOXIN SIMULTANEOUSLY

摘要

PURPOSE: A recombinant multiplex PCR primer for detecting emetic toxin and diarrhetic toxin of Bacillus cereus is provided to accurately and quickly detect both of toxins simultaneously. CONSTITUTION: A multiplex PCR primer is recombined to detect emetic toxin and diarrhetic toxin simultaneously. The multiplex PCR primer is: a forward primer of sequence number 1 and a reverse primer of sequence number 2; a forward primer of sequence number 3 and a reverse primer of sequence number 4; a forward primer of sequence number 5 and a reverse primer of sequence number 6; a forward primer of sequence number 7 and a reverse primer of sequence number 8; a forward primer of sequence number 9 and a reverse primer of sequence number 10; or a forward primer of sequence number 11 and a reverse primer of sequence number 12. A kit for detecting the toxin contains the primer.