TRANSGENIC DEMENTIA ANIMAL OF ALZHEIMER TYPE EXPRESSING δTAU GENE AND METHOD FOR PREPARING THE SAME

摘要

PURPOSE: A method for preparing a transgenic dementia animal of Alzheimer type is provided to express human delta-tau protein in cerebral cortex, hippocampus, and bovine brain and to enhance hyperphosphorylation and agglomeration of tau protein. CONSTITUTION: A vector for transformation contains BAI1-AP4 promoter and human delt-tau gene encoding 1-421th amino acid region cutting between 421th(aspartic acid, Asp421) and 422th amino acids in tau protein C-terminal. BAI1-AP4 promoter is a promoter which specifically expresses in cerebral cortex and Hippocampus region of a mouse. A transgenic dementia animal(deposit number: KCTC11622BP) of Alzheimer type is prepared by introducing the vector. A method for preparing the animal comprises: a step of constructing the vector; a step of injecting the vector to a fertilized egg; and a step of performing implantation of the fertilized egg to an animal.