发明名称 |
BIOSENSOR BASED ON MICROALGAE CELLS FOR DETECTING HEAVY METALS AND HERBICIDES IN AQUEOUS SYSTEMS |
摘要 |
FIELD: chemistry. ^ SUBSTANCE: biosensor contains photo-autotrophic microalgae cells, the fluorescent characteristics of the photosynthesis system of which vary in the presence of cytotoxic chemical compounds in their surroundings: heavy metal ions and herbicides. Cells of green and diatomic microalgae are immobilised in cryogenic gel of polyvinyl alcohol: a cell suspension is deposited on a surface and the cells enter macropores of the polymer carrier under the effect of centrifugal force (5000-14000 g) for 1-10 minutes. A highly sensitive and stable biosensor is obtained based on components taken in the following ratio in wt %: microalgae cells 0.015-1.1; polyvinyl alcohol 7-15; aqueous phase - up to 100. Low concentrations of heavy metals and herbicides in aqueous systems are determined at flow rate of up to 360 ml/h based on the change in the value of relative variable fluorescence chlorophyll cells in the biosensor. The biosensor can be used for a maximum of 60 days. ^ EFFECT: high sensitivity of the biosensor. ^ 2 dwg, 5 ex |
申请公布号 |
RU2426779(C1) |
申请公布日期 |
2011.08.20 |
申请号 |
RU20090141878 |
申请日期 |
2009.11.13 |
申请人 |
ROSSIJSKAJA FEDERATSIJA, OT IMENI KOTOROJ VYSTUPAET MINISTERSTVO OBRAZOVANIJA I NAUKI ROSSIJSKOJ FEDERATSII;GOSUDARSTVENNOE UCHEBNO-NAUCHNOE UCHREZHDENIE BIOLOGICHESKIJ FAKUL'TET MOSKOVSKOGO GOSUDARSTVENNOGOUNIVERSITETA IMENI M.V.LOMONOSOVA |
发明人 |
EFREMENKO ELENA NIKOLAEVNA;KHOLSTOV ALEKSANDR VIKTOROVICH;VORONOVA ELENA NIKOLAEVNA;KONJUKHOV IVAN VLADIMIROVICH;POGOSJAN SERGEJ IOSIFOVICH;RUBIN ANDREJ BORISOVICH |
分类号 |
C12N1/12;C12N11/02;C12Q1/06;G01N33/18 |
主分类号 |
C12N1/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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