METHOD FOR PRODUCING CELL GROWTH FACTORS FROM ADIPOSE-DERIVED STEM CELLS AND MONOCYTES AND APPLICATIONS THEREOF

摘要

The present invention relates to cell growth factors from adipose-derived stem cells and monocytes; and more specifically, to a preparation method of a culture for the adipose-derived stem cells, comprising: (a) culturing adipose-derived stem cells from a stromal vascular fraction (SVF); (b) mixing the adipose-derived stem cells with monocytes and culturing in a serum-free medium; and (c) isolating the culture of the cells and floats from the cell culture medium. Compared to the single culture of adipose-derived stem cells, the culture according to the present invention makes it possible to obtain a high concentration of cell growth factors from a mixed culture of adipose-derived stem cells and monocytes. As such, the equivalent effects such as the tissue generation induced by the cells can be achieved, and any side effects associated with the onset of a tumor that can be caused by the direct administration of adipose-derived stem cells can be avoided, thereby making the present invention method useful for the preparation of a composition for use in tissue generation and skin improvement.