RECOMBINANT NUCLEIC ACID MOLECULE ENCODING FUSED PROTEIN INCLUDING ANTIGEN AND BACTERIAL SECRETION SIGNAL POLYPEPTIDE, EXPRESSION CASSETTE AND BACTERIA, AND METHOD FOR USE THEREOF

摘要

PROBLEM TO BE SOLVED: To provide novel methods for optimizing the efficiency of heterologous protein expression and secretion to maximize the potency of Listeria-based vaccines and other bacteria-based vaccines.

SOLUTION: The present invention relates to recombinant nucleic acid molecules, expression cassettes, and vectors useful for expression of polypeptides, including heterologous polypeptides, such as antigens, in bacteria. Some of the recombinant nucleic acid molecules, expression cassettes and vectors comprise codon-optimized sequences encoding the polypeptides and/or signal peptides. Some of the recombinant nucleic acid molecules, expression cassettes, and expression vectors comprise sequences encoding non-Listerial and/or non-secA1 signal peptides for secretion of the polypeptides. The invention also provides bacteria comprising the nucleic acid molecules, expression cassettes, and expression vectors, as well as compositions such as vaccines comprising the bacteria.

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