METHOD FOR CONTROLLING PH, OSMOLALITY AND DISSOLVED CARBON DIOXIDE LEVELS IN A MAMMALIAN CELL CULTURE PROCESS TO ENHANCE CELL VIABILITY AND BIOLOGIC PRODUCT YIELD

摘要

PURPOSE: A method for controlling osmorality and dissolved carbon dioxide level is provided to improve cell growth, survival rate, and density. CONSTITUTION: A method for improving yield of a product in a process for culturing mammalian cells comprises a step of removing dissolved carbon dioxide through surface gas exchange from the surface of a culture medium for cells. The osmorality of the medium is maintained in an optimal range for special cells during a mammalian cell culture process.