摘要 |
A method of finding a biocatalyst having ammonia lyase activity, in particular a lysine ammonia lyase, comprising - providing a library comprising a plurality of cells in one or more cell cultures, which cells are candidates for having an ammonia lyase activity, which one or more cultures comprise a culture medium containing at least one nitrogen source selected from the group of amines, including amino acids, and structural analogues thereof, as sole nitrogen source or sources for the cells; - selecting at least one candidate which grows in said culture medium; and - screening the selected candidate or candidates which grow in said culture for the ammonia lyase activity. |
申请人 |
DSM IP ASSETS B.V.;RAEMAKERS-FRANKEN, PETRONELLA CATHARINA;MEURER, GUIDO;ECK, JUERGEN WOLFGANG;LIEBETON, KLAUS JUERGEN;TREFZER, AXEL CHRISTOPH |
发明人 |
RAEMAKERS-FRANKEN, PETRONELLA CATHARINA;MEURER, GUIDO;ECK, JUERGEN WOLFGANG;LIEBETON, KLAUS JUERGEN;TREFZER, AXEL CHRISTOPH |