Polynucleotide comprising sequences of wheat gliadins and use thereof for silencing by RNAi

摘要

The present invention relates to the specific silencing of theα(alpha),β(beta),γ(gamma) andω(omega)-gliadins of hard wheat for flour by RNA interference (RNAi) through employment of a polynucleotide which is transcribed into an hpRNA (hairpin RNA). Furthermore the present invention additionally relates to a vector, cell, plant or seed comprising the polynucleotide, the expression whereof is specifically directed in particular tissues of wheat seeds through gene expression-regulating sequences such as, for example, the promoter of a gene ofγ-gliadins or the promoter of the gene encoding for a D-hordein.