METHOD FOR INCREASING TRANSFORMATION EFFICIENCY OF CHINESE CABBAGE PLANT USING AGROBACTERIUM AND CHINESE CABBAGE PLANT PRODUCED BY THE METHOD

摘要

PURPOSE: A method for enhancing transformation efficiency of Brassica campestris L. using Agrobacterium is provided to establish stable transformation system of high efficiency. CONSTITUTION: A method for enhancing transformation efficiency of Brassica campestris L. comprises: a step of performing dark germination of Brassica campestris L. seeds at 24-26°C for 3-5 days; a step of culturing the hypocotyl part of Brassica campestris L. in a MS medium containing 0.5-1.5 mg/L of NAA and 3-5 mg/L of BA at 22-24°C for 1-3 days by photo-cultivation; a step of suspending Agrobacterium tumefaciens containing a recombinanat plant expression vector to a medium; a step of dipping the hypocotyl part of Brassica campestris L. in the prepared strain-inoculated medium for 5-15 minutes; a step of culturing the hypocotyl part of Brassica campestris L. in a medium I containing NAA, BA, AgNO_3, and antibiotics for 2-3 months to induce a shoot; and a step of culturing the shoot in a medium II.