发明名称 |
ARTIFICIAL CHROMOSOME VECTOR |
摘要 |
According to the present invention there is provided a method of producing a protein in a eukaryotic cell line, comprising the steps of a) providing a backbone of an artificial chromosome, b) recombining the nucleic acid encoding said protein into said backbone to generate an expression vector, c) introducing said expression vector into a eukaryotic host cell line to obtain a eukaryotic expression cell line, d) cultivating said expression cell line to produce said protein, and e) isolating said protein. The invention further relates to respective vectors and transgenic cell lines. |
申请公布号 |
EP2356241(A1) |
申请公布日期 |
2011.08.17 |
申请号 |
EP20090756728 |
申请日期 |
2009.11.18 |
申请人 |
BAUER, ANTON;CASANOVA, EMILIO;AUSTRIA WIRTSCHAFTSSERVICE GESELLSCHAFT MBH |
发明人 |
BLAAS, LEANDER;BAUER, ANTON;CASANOVA HEVIA, EMILIO MANUEL |
分类号 |
C12N15/79 |
主分类号 |
C12N15/79 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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